Coming in 2011:The Optical Micro-Spectroscopy System (OptiMiS™) Version 1.0

OptiMis system on a Nikon Inverted Scope at University of Wisconsin Madison

The OptiMiS™ system has enabled researchers to study phenomena in living cells that no other system can duplicate.

The system produces spectrally resolved fluorescence images of living cells using a multi-photon microscope (MPM) after only one full scan of the sample. Thus, it allows complete spectral resolution at high scanning speeds. This new capability enables the following applications including the following:

Determination of the Supra-molecular Structure of protein complexes in living cells by employing the principles of Fluorescence Resonance Energy Transfer (FRET);


The OptiMiS™ system will be sold as an accessory to be added to existing or new commercial laser scanning multiphoton microscopes. It has three components: the spectral resolution device and scanner that are designed to interface with existing microscopes, the electron-multiplying CCD (EMCCD) camera sold by multiple vendors, and the software controlling the scanner on the microscope and providing analysis of the data from the CCD camera.


The OptiMiS™ system allows the following:

  1. Determination of the entire spectral fingerprint of the chemical species contained in a small sample voxel at once for all wavelengths investigated, thereby allowing one to perform molecular imaging;
  2. High efficiency capture of the dispersed light allowing detection of low levels of fluorescence;
  3. Single-photon detection allowing greater sensitivity of detection
  4. Rapid acquisition speed;
  5. Overall design simplicity, thereby increasing the robustness in system operation and reliability