OptiMiS encapsulates technology developed by Dr. Valerica Raicu’s team at University of Wisconsin-Milwaukee after 7 years of research. When used as an accessory to a multi-photon microscope system (MPM), it provides a unique blend of speed, efficiency and spatial and spectral resolution to microscopists. Perhaps more importantly, OptiMiS can upgrade imaging capability of almost any microscope to achieve MPM-grade spectral resolution with the addition of a femtosecond laser. It addresses limitations of existing image-based analyses which obtain multicolor analysis after multiple scans over a period of many minutes which may provide misleading information about complex intracellular dynamics with respect to protein trafficking and transient structural aggregations.

Unique Features and Benefits

Single Scan Image Capture: No need for acceptor photobleaching or multiple scans
Superbly Resolved Intracellular Images: Delivers spectral resolution as low as 1.25 nm per channel
Unsurpassed Detection Capability: Provides spectral information with single molecule/molecular complex sensitivity
Ultra-fast Data Acquisition: Collects whole spectrum for each pixel within microseconds
Proven Technology: Quantitative FRET imaging utilized in several publications on determination of binding stoichiometry and geometry of protein complexes
Highly Accommodating Wavelength Range: Allows easily resolvable donor/acceptor fluorophore pairs
Flexibility to Use and Upgrade Present Systems: Upgrade MPM or any other microscope with a side port
Freely Available Analysis Software: Spectral unmixing and FRET efficiency calculations at single pixel level


Live cell imaging techniques are providing the first detailed look at intracellular interactions with fresh insights into molecular functions and dynamic structural associations. To fully engage in intelligent drug design based on biochemistry and macromolecular structural analysis rather than random high throughput screening, there is a clear need to unlock intracellular interactions through more sensitive and efficient imaging tools. A wide range of standard microscopy applications can be enhanced technically by the unique capabilities offered by OptiMiS. These include:

FRET-Based Applications:

  1. Detecting Protein-Protein Interactions
  2. Studying Conformational Changes of Macromolecules (Proteins and Nucleic Acids)
  3. Measuring Intracellular Concentrations

Non-FRET-Based Applications:

  1. Quantifying Biomolecular Relationships: Protein co-localization
  2. Localization of Specific DNA/RNA Sequences: Fluorescence in-situ hybridization (FISH)

Instrument Specifications

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